The laser line clean up filters used in the instrument assures the noise level in the laser output to bare minimum. The Optical paths are carefully designed that the lasers can be used independently without changing any Opto-mechanical or Optics component in the path. Lenses, Mirrors etc are carefully chosen to minimize any aberration or any loses.
Dichroic filters, band pass filters, emission filters etc are sourced from best suppliers in the world for matching the laser output and sample data acquisition requirements.
High NA (1.20), water immersion Microscope Objective is used for microscopy. These super apochromat objectives fully compensate for both spherical and chromatic aberrations from the UV to the near infrared region. Their sensitivity to fluorescence emissionensures the acquisition of sharp, clear images, without color shift, even in bright field and Nomarski DIC observations.
We have used the Single Photon Counting APD for detection of fluorescence. It detects single photons of light over the 400 nm to 1060 nm wavelength range - a range and sensitivity that far outperforms a photomultiplier tube. The fiber-coupled Single Photon Counting APD used has a peak photon detection efficiency of more than 65% at 650nm over a 180μm diameter. The photodiode is both thermoelectrically cooled and temperature controlled, ensuring stabilized performance despite ambient temperature changes.