Confocal Raman spectroscopy is a powerful analytical technique that combines Raman spectroscopy with confocal microscopy. It allows spatially resolved chemical analysis of samples by providing high-resolution Raman spectra from specific locations within a sample. A focused laser beam is used to illuminate the sample using the microscope, and the Raman-scattered light is collected through the same objective lens and coupled to the spectrometer.
Holmarc CRM 216C is a fully automatic inverted infinity confocal microscope integrated with a high-resolution spectrometer and laser excitation system. The new ergonomic design helps the user capture the microscopic image and Raman spectrum easily. The color-corrected infinity design microscope has a motorized turret, filter wheel, switching mirror, sample stage, and focusing features. The turret comes with six slots for holding six different magnification microscope objectives. It is also equipped with an 8-slot automatic filter wheel for dielectric beamsplitters and a notch/edge filter combination for Raman and photoluminescence applications with different wavelength lasers. The viewing head is a stereo binocular head with a 5.0 MP CMOS camera, which gives clear, sharp, high-resolution images of the sample. The coaxial illumination and bottom LED illumination can be controlled by software. The hand-held controller provided with the system makes easy adjustments of the sample stage and LED control by using the joystick key.
The spectrometer is utilizing a Czerny Turner configuration with a motorized grating turret. The aberration-corrected 400-mm focal-length parabolic mirrors are provided for focusing and collimating the light. The high-reflective, aluminum-coated mirrors that are used inside the spectrometer and microscope provide robust performance. The dispersion element is a holographic diffraction grating that is balanced for the stoke wavelength to get maximum diffraction efficiency. The variable slit with a micrometer provided at the entrance port makes the slit width adjustment easy. The highly sensitive cooled linear CCD array detector is used as the detector, and the exposure time can be adjusted in the software from several milliseconds to minutes. The encoder-based grating rotation achieves better resolution and repeatability throughout the entire wavelength range.
The system comes with a standard 532 nm low-noise, 0.06 nm spectral resolution, and a high-stability laser with a Raman filter set for the same. It also has the facility to keep two more lasers inside the spectrometer, and the selection can be done by a motorized stage. Single, double, and triple grating turret configurations are also available.
The dedicated micro-Raman software features single, multi-spectrum, multi-live view, and Raman mapping of samples. A single shot can capture a Raman spectrum range of 100 cm1 to 3500 cm1. The spectrometer wavelength can be changed to cover a spectrum larger than 3500 cm1.
Photoluminescence measurement can be performed with the stitched scanning option, which covers the entire wavelength range of 200 nm to 1050 nm (depending on the grating and detector).
| Microscope | ||
| Equipment type | : | Inverted |
| Optical System | : | Colour Corrected Infinity Optical System |
| Sample Stage | : | Stepper motor controlled |
| Objectives | : | 10x, 20x, 40 x and 60x Plan objectives |
| 45 mm parfocal confocal objective lens | ||
| Objective Turret | : | Motorized positioning and 6 slots for keeping |
| Filter Selection Unit | : | 8 - slot dichroic filter cube assembly |
| Tube Length | : | 200 mm |
| Condenser | : | Abbe condenser |
| Focus | : | Motorized Stepper motor controlled |
| Illumination for imaging | : | Bottom and Co-Axial HB-LED Based |
| Illumination Control | : | Continuously Variable PC-controlled |
| Fully automatic Upright infinity confocal microscope and spectrometer design |
| Easy sample focusing and Raman / PL analysis |
| 6 slot Motorized turret for microscope objective |
| 8 slot automatic filter wheel which can accommodate standard 35 x 25 mm & 25 mm diameter dielectric & edge / notch filters |
| High Resolution 400 mm FL Czerny - Turner Spectrograph with 0.95 cm-1 resolution |
| Optional Double / triple turret grating system |
| Wide wavelength range 190nm - 2400 nm (depends on grating & detector) |
| Cooled CCD detector with 3648 pixels |
| 3 different lasers add on provision with automatic selection |
| Motorized sample stage X Y & Z |
| Raman mapping |
| Solid, liquid & thin film sample analysis |
| Nanotechnology |
| Battery & solar cell research |
| Material science |
| Petroleum industry |
| Forensics |
| Life science |
| Pharmaceutical Industry |
| Bio-medical |
| Chemical analysis |
| Environment science |
| Gemology |
Cyclohexane
Graphite
Silicon & Graphite
Silicon
MoS2 On Silicon
Exfoliated Graphene
| Laser | |
| Laser wavelength | 532 nm |
| Spectral Line Width | 0.06 nm |
| Type | Low noise variable power 100mW DPSS Laser |
| Shutter | Electronic |
| Option for two additional lasers | |
| Photoluminescence & Upconversion Spectrometer | |
| Spectrometer Type | Czerny-Turner |
| Focal Length | 400 mm |
| Focal Ratio | f / 4 |
| Spectral range | 350 - 1050 nm |
| The spectral resolution | 0.1 nm |
| Raman | |
| Laser wavelength (Std) | 532 nm |
| Optional lasers | Support up to 3 lasers in combination with a motorized selection option |
| External Laser Integration | Additional port for integrating the external lasers |
| Shutter | Electronic Type |
| Spectrometer Type | Czerny-Turner |
| Grating mount | Turret type |
| Grating | 600 l / mm (Optional 1200 / 1800 / 2400 l / mm) |
| Focal Length | 400 mm |
| Raman Spectral range | 100 cm-1 - 3500 cm-1 ( up to 7000 cm-1 on stitching) |
| The spectral resolution | 0.95 cm-1 |
| Raman Filter set details | |
| Razor Edge Dichroic laser beam splitter | |
| Raman Edge Filter Specifications : | |
| Transmission Band 1 | Tavg > 93% |
| Blocking Band | ODabs > 6 |
| Edge Steepness (%) | 0.2 % |
| Edge Steepness (cm-1) | 37.5 cm-1 |
| Edge Steepness (nm) | 1.1 nm |
| Transition Width (nm) | 2.7 nm |
| Transition Width (cm-1) | 90 cm-1 |
| Mapping Stage | |
| Mapping sample stage | XYMR50 Motorized Stepper motor controlled |
| Travel | 50 x 50 mm |
| Resolution | 0.3125 Micron |
| Scanning Speed | 4 mm / sec |
| Focusing | Motorized Stepper motor controlled |
| Travel | 25 mm |
| Resolution | 0.1 Micron |
| Controller | HOLMARC XYZ Stepper controller Unit |
| Scanning | Programmable |
| Joystick | 3 Axis Joystick controller |
| Detector | |
| Detector Unit | Cooled Linear CCD Array (16Bit) |
| Number of Pixels | 3648 pixels |
| Pixel Size | 8 um x 200 um |
| Sensor Length | 29.18 mm |
| Pixel Output Clock | 0.5 MHz |
| Data Storage on Camera | 4 Frames |
| ADC Resolution | 16 bits |
| Exposure Time Range | 1 ms - 10,000 ms |
| Frame Rate | 138 scans / second* |
| Trigger | Yes (Optional) |
| Interface | USB 2.0 |
| Imaging Camera Specifications | |
| Optical format | 1 / 2.5” CMOS |
| Active imager size | 5.70 mm x 4.28 mm, 7.13 mm diagonal |
| Active pixels | 2592 x 1944 (5Mp) |
| Pixel size | 2.2 x 2.2 um |
| Color filter array | RGB Bayer pattern |
| Shutter type | Global reset release (GRR), Snapshot only, Electronic rolling shutter (ERS) |
| Maximum data rate / master clock | 96 Mp / s at 96 MHz (2.8 V I / O) |
| Frame rate | Full resolution - Programmable up to 15 fps |
| VGA (640 x 480 with binning) - programmable up to 70 fps | |
| ADC resolution | 12 - bit, on-chip |
| Pixel dynamic range | 70.1 dB |
| Operating temperature | -30°C to +70°C |
| Software | Holmarc Micro Raman Spectrometer PRO |
| # System Control and Data Collection software including Software for Sample Viewing |
| # Capable of managing user-built libraries and material identification with spectral databases |
| Optional : |
| Lasers : 488 nm, 633nm, 785 nm etc. |
| Spectrometer detector : back thinned cooled CCD, EMCCD etc. |
| Sample characterization : Temperature controlled sample stage, polarization kit, etc |
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