Holmarc’s Digital In-line Holography microscope, model HO-DIHM-HT01, is a powerful imaging device that allows for the digital reconstruction of complex objects (amplitude and phase) from a single intensity image captured by passing coherent light through it.
Holmarc’s DIHM is equipped with a transmission type in-line geometry. A coherent point light source illuminates the sample, and a CMOS camera records the 2D interference pattern created by the scattered light (object beam) with non-scattered light (reference beam). The software then computes the field backward to different planes to numerically recreate the samples’ 3D structure from the recorded 2D interference pattern. For 3D reconstruction, the software employs a single shot phase unwrapping algorithm. The DIHM’s broad depth of field allows it to image large volumes with equivalent resolution and to image particles at different depths simultaneously without the use of mechanical scanning.
The HO-DIHM-HT01 can be used both in-situ and ex-situ to image particles ranging in size from sub-microns to several millimetres. It records the interference pattern using a 405nm diode laser with pinhole assembly as a point light source and Sony’s 1/1.8” 3.17Mpix CMOS IMX174 sensor. The 134 fps USB3.0 sensor delivers great image quality and low noise performance. The distance between the sample to pinhole and sample to camera is adjustable from the software. A motorized ND filter wheel is provided in front of the laser to adjust the laser power for different sample conditions. DIHM_V01 is an easy-to-use programme that allows you to save, rebuild, and analyse each hologram frame.
DIHM configuration | Horizontal Laser |
Light source | 405nm diode laser (50mW) |
Sample illumination beam profile | Plane wave front (Collimated beam) |
Intensity control | Motorized variable ND filter, via software |
Spatial filter assembly | Microscope objective based |
Collimating optics | Multi-element design, Spherical aberration corrected |
Source to sample distance | Fixed |
Sample to Camera distance | Adjustable up to 200 mm, motorized movement, adjustable via software |
YZ Sample stage | Manual, Provision to hold optical chamber of 8cm x 4cm x 1cm or higher |
Camera specifications | |
Sensor type | CMOS Mono |
Sensor size | 14.158 mm x 7.500 mm (16.02 mm diagonal) |
Pixel size | 3.45 um Full resolution = 4104 x 2174 Pixels (8.92 Mpix) |
Shutter | Global shutter |
Sensor characteristic | Linear Readout mode = Progressive scan |
Sensor model | Sony IMX267 |
ADC | 12 bit |
Gain (Master / RGB) | 24X / 4X |
Pixel clock range | 99 MHz - 395 MHz |
Frame rate freerun mode | 33 |
Frame rate trigger (Continuous) | 33 |
Frame rate trigger (maximum) | 33 |
Exposure time (Min - Max) | 0.027 ms - 1000 ms |
Long exposure (Maximum) | 30000 ms |
Power consumption | 1.6 W - 3.9 W |
Image memory | 128 MB |
Interface connector | USB Type-C, Screwable |
Power supply | USB cable |
Lens mount | C - Mount |
Dimensions | 34 mm x 44 mm x 47 mm |
Software | DIHM_V02 |
Software features | Image grabbing, Continuous mode recording, Frame by frame processing, Movie mode processing, image measurements, motorized stage controls |
Fig. 1. Hologram - Single frame
Fig. 2. Binary particle map
Particle 1 - Amplitude
Particle 1 - Phase
Particle 1 - 3D Phase
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Particle 2 - Amplitude
Particle 2 - Phase
Particle 2 - 3D Phase
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Particle 3 - Amplitude
Particle 3 - Phase
Particle 3 - 3D Phase
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Particle 4 - Amplitude
Particle 4 - Phase
Particle 4 - 3D Phase
-
Particle 5 - Amplitude
Particle 5 - Phase
Particle 5 - 3D Phase
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Particle 6 - Amplitude
Particle 6 - Phase
Particle 6 - 3D Phase
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